Cloned (Comment) | Organism |
---|---|
gene nmagghr, a single gene, synthesis of a cDNA from the total RNA extracted from Natrialba magadii strain ATCC 43099, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli | Natrialba magadii |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in apoform, mixing of 0.5 ml of 6.6 mg/ml protein in 50 mM Tris-HCl, pH 7.5, and 3.5 M NaCl, with 0.5 ml of reservoir solution containing 25% w/v PEG 3350, 0.1 M Tris-HCl, pH 8.5, and 0.2 M lithium sulfate, at 21°C, X-ray diffraction structure determination and analysis at 2.61 A resolution, molecular replacement using the structure of monomeric putative GST from Corynebacterium glutamicum (PDB ID 3M1G) as a search model, model building | Natrialba magadii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
recombinant N-terminally His6-tagged enzyme, gel filtration | Natrialba magadii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii ATCC 43099 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii NBRC 102185 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii DSM 3394 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii CCM 3739 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii NCIMB 2190 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii JCM 8861 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii CIP 104546 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii MS3 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii IAM 13178 | - |
gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii ATCC 43099 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii NBRC 102185 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii DSM 3394 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii CCM 3739 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii NCIMB 2190 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii JCM 8861 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii CIP 104546 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii MS3 | - |
glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | Natrialba magadii IAM 13178 | - |
glutathione disulfide + hydroquinone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Natrialba magadii | D3SS28 | - |
- |
Natrialba magadii ATCC 43099 | D3SS28 | - |
- |
Natrialba magadii CCM 3739 | D3SS28 | - |
- |
Natrialba magadii CIP 104546 | D3SS28 | - |
- |
Natrialba magadii DSM 3394 | D3SS28 | - |
- |
Natrialba magadii IAM 13178 | D3SS28 | - |
- |
Natrialba magadii JCM 8861 | D3SS28 | - |
- |
Natrialba magadii MS3 | D3SS28 | - |
- |
Natrialba magadii NBRC 102185 | D3SS28 | - |
- |
Natrialba magadii NCIMB 2190 | D3SS28 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli | Natrialba magadii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii ATCC 43099 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii NBRC 102185 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii DSM 3394 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii CCM 3739 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii NCIMB 2190 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii JCM 8861 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii CIP 104546 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii MS3 | ? | - |
? | |
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii IAM 13178 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii ATCC 43099 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii NBRC 102185 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii DSM 3394 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii CCM 3739 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii NCIMB 2190 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii JCM 8861 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii CIP 104546 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii MS3 | ? | - |
? | |
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene | - |
Natrialba magadii IAM 13178 | ? | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii ATCC 43099 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii NBRC 102185 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii DSM 3394 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii CCM 3739 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii NCIMB 2190 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii JCM 8861 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii CIP 104546 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii MS3 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii IAM 13178 | gamma-glutamylcysteine glutahione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii | gamma-glutamylcysteine glutathione disulfide + hydroquinone | - |
? | |
gamma-glutamylcysteine + ethacrynic acid | - |
Natrialba magadii | ? | - |
? | |
glutathione + 1,4-benzoquinone | - |
Natrialba magadii | ? | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii ATCC 43099 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii NBRC 102185 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii DSM 3394 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii CCM 3739 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii NCIMB 2190 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii JCM 8861 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii CIP 104546 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii MS3 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Natrialba magadii IAM 13178 | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-hydroxyethyldisulfide | - |
Natrialba magadii | ? | - |
? | |
glutathione + dehydroascorbic acid | - |
Natrialba magadii | ? | - |
? | |
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii ATCC 43099 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii NBRC 102185 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii DSM 3394 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii CCM 3739 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii NCIMB 2190 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii JCM 8861 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii CIP 104546 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii MS3 | ? | - |
- |
|
additional information | the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate | Natrialba magadii IAM 13178 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant N-terminally His6-tagged enzyme, SDS-PAGE | Natrialba magadii |
More | structure analysis of enzyme NmGHR, detailed overview | Natrialba magadii |
Synonyms | Comment | Organism |
---|---|---|
nmagghr | - |
Natrialba magadii |
Nmag_1170 | - |
Natrialba magadii |
NmGHR | - |
Natrialba magadii |
Xi class glutathione transferase | - |
Natrialba magadii |
Xi class GST | - |
Natrialba magadii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | assay at | Natrialba magadii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | assay at | Natrialba magadii |
General Information | Comment | Organism |
---|---|---|
evolution | Xi class glutathione transferases (GSTs) are a group, within this large superfamily of enzymes, specifically endowed with glutathione-dependent reductase activity on glutathionyl-hydroquinone. Enzymes belonging to this group are widely distributed in bacteria, fungi, and plants but not in higher eukaryotes. Xi class GSTs are also frequently found in archaea. Multiple alignment analysis of NmGHR in comparison with other Xi class GSTs show that all sequences display several common motifs. All members exhibit a strictly conserved CPWA motif that could play a key role in the binding of GS-(hydro)-quinones. Phylogenetic analysis, members of prokaryotic and eukaryotic Xi class GSTs and their homologous belonging to different GST classes are characterized by the presence of a cysteine residue in the G-site, overview | Natrialba magadii |
additional information | enzyme NmGHR displays is enriched in negatively charged residues, which are evenly distributed along the surface of the protein, and is characterized by a peculiar distribution of hydrophobic residues. The thiol binding site (G-site) of the enzyme is well suited for hosting gamma-glutamyl-cysteine. Circular dichroism analysis of NmGHR | Natrialba magadii |